FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4745110
Sequences flagged as poor quality0
Sequence length151
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTTAAATGGTCTGTTTGGTAGCGAACAAGGCAAGAGATTCGTTAAAT67290.14180914667942365No Hit
GTATTGGATGTGTTCAAAAGATACCAAGAAGAATATGACAGGTATTTAAA60330.12714141505676368No Hit
TCGTAAAGGTGGTGCAGGCGGTTGGGAACAGCTTTGATGACCGGCCTTAA47610.10033487105672997No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG78500.060.7847752
GAGCAAA95900.050.9149931
CGAAAGC102250.047.5719873
GCAAAAG119750.040.2567023
GAAAGCA130600.037.3543474
AGCGAAA132700.036.2486531
AGCAAAA137450.035.6648642
AAGCAGG180200.035.5596586
CAAAAGC145250.033.7863854
AGCAGGG92050.031.9711117
AAAGCAG221000.031.6190935
AGCAGGC58450.025.7941118
ATGTGTT41700.025.3780698
TGGATGT46850.023.208465
AAAAGCA220150.023.0156445
GGATGTG50900.021.3615886
ATTGGAT60150.018.8010063
GCAGGGG44450.018.7528748
TAGGCCG4303.7587597E-818.5425979
GCAGGGC45450.017.8618288
Produced by FastQC (version 0.11.5)