FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37224691
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13005763.493853045012516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA162000.067.4499051
GAGCAAA798050.063.1811751
CGAAAGC299500.058.2775963
GCAAAAG913200.055.5738563
AGCAAAA1026150.049.4537622
GCAGGGT344500.044.984959
CAAAAGC1164950.043.898714
AGCAGGG628650.042.371778
GCGAAAG421750.041.5598872
AAGCAGG1508000.039.8237347
AAAGCAG1681550.036.6213236
AAAAGCA1491100.034.737815
GCAGGTA353800.033.6486369
AGCGAAA521700.033.397661
ACAAGGG330850.030.4862733
GAAAGCA680950.026.5660784
AGCAGGC278300.025.1685738
GGGTGTT320950.023.4455387
TAGAAAC532250.023.4352862
AACAAGG749450.023.0113752
Produced by FastQC (version 0.11.5)