FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7720001
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCG265400.34378233888829807No Hit
CTAGTGATCCGGCCATGGCACGTGGGTGCGTGGTCGCTCAACGGATAAAA157370.2038471238539995No Hit
GAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCGTGGTC155740.20173572516376617No Hit
TACTAGGGACGGCCCTTCTCAAATTTCCTGCGCGCACAGAGGATAGGGAC155500.20142484437502017No Hit
GGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACG114060.14774609485154214No Hit
TAGCCAACCAGCCATGCACCTGGCGGTACAACTGGCACACCAGAGGTTCG107030.13863987841452352No Hit
GATAAAAGGTACCCCGGGGATAACAGGCTGATCTTGCCCAAGAGTCCATA96520.12502589054068775No Hit
GTATGGAGGATGGCGTAATCGTAAGATGCTTGGCTTCTATGAAAATCTTT93660.12132122780813112No Hit
GTTTAATTTTGAGGATGGTTATTTTTATCCAGCTAAGGAAAGCATCGATA91970.11913210892071129No Hit
AAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCGAACGT90990.11786267903333174No Hit
GAACTAGTGATCCGGCCATGGCACGTGGGTGCGTGGTCGCTCAACGGATA84360.10927459724422316No Hit
GGTCAATACAACCCCATGTTGTGTAGCCCCAAAGCTCTACACCATCTTCA80000.10362692958200395No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA222800.084.332331
AAAAGCA289500.065.45395
GCAAAAG290600.065.0314563
AGCAAAA295600.063.6832542
CAAAAGC337000.056.3142744
AAGCAGG382450.055.659447
CGAAAGC104700.053.6091583
GCGAAAG107900.051.8824922
GCAGGCT115950.049.7740069
AGTAGAA88350.049.5760881
GAGCGAA74450.049.0916521
AGCAGGC167950.045.8460548
AAAGCAG514500.043.9338156
AGCGAAA132050.042.0660971
GGTCGAT22150.040.5966151
ACAAGGC71600.035.340278
GTAGAAA142750.034.798161
ATTTCCG21800.032.923656145
TAGTGCG21050.032.721187
AGTGCGC21950.031.0492278
Produced by FastQC (version 0.11.5)