FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813809
Sequences flagged as poor quality0
Sequence length151
%GC60

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCGCCGATGCGGG87511.0753137406934552No Hit
AAGCAGGTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCGCCGAT79440.9761504235023206No Hit
AGCGAAAGCAGGTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCG68150.8374200825992341No Hit
CAGGAAGACAATCGTGTTCGCAACGTCTTCGGGCGTACCGACGCGTCCGA58730.7216681063984302No Hit
GTCTTCGGGCGTACCGACGCGTCCGAGCGGATGAAACGAATCGAGCGCTT49610.6096024988664416No Hit
AGCGAAAGCAGGAAGACAATCGTGTTCGCAACGTCTTCGGGCGTACCGAC37790.4643595733151145No Hit
GAAGACAATCGTGTTCGCAACGTCTTCGGGCGTACCGACGCGTCCGAGCG36890.4533004673086682No Hit
AGACAATCGTGTTCGCAACGTCTTCGGGCGTACCGACGCGTCCGAGCGGA36890.4533004673086682No Hit
AAAGCAGGGCGAGCGAGCGCACCACCCGCTGAATGCCGGTGCCTGCATCA34420.4229493652687547No Hit
TTCCAGGGCAGCCTCACCGGGATGGACAACCTGCGGTTCATCTGCCGCAT34190.42012314928932953No Hit
AGCGAAAGCAGGGCGAGCGAGCGCACCACCCGCTGAATGCCGGTGCCTGC33460.41115298552854534No Hit
GAAAGCAGGTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCGCCG33150.4073437379041028No Hit
GTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCGCCGATGCGGGT30140.3703571722603215No Hit
ATCGTGTTCGCAACGTCTTCGGGCGTACCGACGCGTCCGAGCGGATGAAA28360.3484847181586834No Hit
GCCCTGGAACGCACCGCCGAATGCGAGCGGCCACGACACGCTCATGCTGC26570.3264893851014182No Hit
TTACTGGGGCGATTGAGCTGGAGTTGGGCCGGCACGGCGCCAACTCCGGT26340.323663169121993No Hit
GCTTTATCTGGAACGCCTGATTCAGCCGAATACCCCCGACGTTGCAGTCG25750.31641331073998935No Hit
GCAGGAAGACAATCGTGTTCGCAACGTCTTCGGGCGTACCGACGCGTCCG24600.3022822308428636No Hit
CTCACGCGCAATCTCGCGATTGAACTCGCCGATGCGGGTATCCGGGTGAA23200.2850791770550584No Hit
ATCGATGGCGATCGAATTTGACTGCTTCCTCATCGACGAAATCATTGCGG22380.2750031026936296No Hit
GGGCATGATCGCGTGGGGCGTTCTGTCGCTTTTGTTGGCGGGCCTGCGCG21980.27008794446854234No Hit
GAATAGATGCCCGGGATCGGACTGCAAACATACCGGAGTTGGCGCCGTGC19980.2455121533431063No Hit
TCTATGGCGTCAATGTCAAAGATGCCGAACCGTTCGTACAAGAGTTCTCG19830.24366896900869858No Hit
GTATGCGTGCGCGCCTCGCGTTCGCGTTATCGATGGCGATCGAATTTGAC19540.24010547929551035No Hit
GCACGCGCTCACGCGCAATCTCGCGATTGAACTCGCCGATGCGGGTATCC19300.23715638436045805No Hit
GACAATCGTGTTCGCAACGTCTTCGGGCGTACCGACGCGTCCGAGCGGAT19280.2369106264492037No Hit
GGTATGCGTGCGCGCCTCGCGTTCGCGTTATCGATGGCGATCGAATTTGA18150.22302530446333232No Hit
GTATTTGCGCTGGGCATGATCGCGTGGGGCGTTCTGTCGCTTTTGTTGGC18000.22118212012892458No Hit
CCCCAGTAACGCACGCTCAATAGTTGAATTCGGATGATCGAACTCAAGAA17800.218724541016381No Hit
CTCAAGAACATCGCCAAAGACTACTACACGCGACAGGGCCGACGCAGGGT17160.21086028785624145No Hit
CATCTATGGCGTCAATGTCAAAGATGCCGAACCGTTCGTACAAGAGTTCT17070.20975437725559684No Hit
ATGATTGACACGTCGACGAGCAACTGGCGTTCCCGCGACGCGGGCGCTGA17060.20963149829996966No Hit
GATTGACACGTCGACGAGCAACTGGCGTTCCCGCGACGCGGGCGCTGATT16590.20385618738549216No Hit
CTTTGACATTGACGCCATAGATGCGGCAGATGAACCGCAGGTTGTCCATC16300.20029269767230395No Hit
GTTCTTGAGTTCGATCATCCGAATTCAACTATTGAGCGTGCGTTACTGGG16220.19930966602728653No Hit
AAAGCAGGCCGACCATCATCGCCTGGTGCGCGAGGGTCATCACTTGTTCG16120.1980808764710147No Hit
ATGTCAAAGATGCCGAACCGTTCGTACAAGAGTTCTCGGAACTGGGCTAC16040.19709784482599724No Hit
AGCAGGAAGACAATCGTGTTCGCAACGTCTTCGGGCGTACCGACGCGTCC15620.19193692868965567No Hit
GCGAAAGCAGGTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCGC15490.19033950226650234No Hit
TGTTTGACGAGATCGAAGAGGCCTACGCGTTCTACCAGGAAAACAGCGCC15230.18714464942019565No Hit
GGAAGACAATCGTGTTCGCAACGTCTTCGGGCGTACCGACGCGTCCGAGC14930.18345828075138024No Hit
ATCTGGAACGCCTGATTCAGCCGAATACCCCCGACGTTGCAGTCGAACCC14820.18210661223948124No Hit
ACTATTGAGCGTGCGTTACTGGGGCGATTGAGCTGGAGTTGGGCCGGCAC14750.18124645955009097No Hit
AGCGAAAGCAGCTTTATCTGGAACGCCTGATTCAGCCGAATACCCCCGAC14640.179894791038192No Hit
CCATAGATGCGGCAGATGAACCGCAGGTTGTCCATCCCGGTGAGGCTGCC14600.17940327521568328No Hit
AGCGAAAGCAGGCCGACCATCATCGCCTGGTGCGCGAGGGTCATCACTTG14600.17940327521568328No Hit
CACCACAACTGAGCCGCGGTTCATACGGCATCGCCGCGCGACATTTGTCG14360.17645418028063095No Hit
CGTCAAACATGCGCAACCGGCCTTGCACCAGCACGGCTGCCCGAGTGCAA14280.17547114863561353No Hit
GATGAAACGAATCGAGCGCTTTCATCGCACCTGCAATGTCTTCCTTCGCC14270.17534826967998632No Hit
ACAATCGTGTTCGCAACGTCTTCGGGCGTACCGACGCGTCCGAGCGGATG14190.1743652380349689No Hit
CTCAATAGTTGAATTCGGATGATCGAACTCAAGAACATCGCCAAAGACTA14190.1743652380349689No Hit
CAATAGTTGAATTCGGATGATCGAACTCAAGAACATCGCCAAAGACTACT14070.17289069056744272No Hit
GTTCGCGGATGTACGCGGCATCGTGGCTGACGATGATGAGGGCTCGATCC13890.17067886936615348No Hit
GTACCGACGCGTCCGAGCGGATGAAACGAATCGAGCGCTTTCATCGCACC13780.16932720085425448No Hit
GGCAAGGACCGCTGACATGGCGCCGTGCCCTCTTTCCGACCCCGCATTCC13690.16822129025360988No Hit
ACCTGCGAGAGCCGGTCAAGAGCTATTCGGCAGGTATGCGTGCGCGCCTC13610.16723825860859243No Hit
GCATCATGTTGCGCAATGATTGACACGTCGACGAGCAACTGGCGTTCCCG13320.1636747688954042No Hit
AAATCATTGCGGTCGGCGACAGCCGGTTTCACGCCAAGTGCCACCACGAA13290.16330613202852265No Hit
GATGTACGCGGCATCGTGGCTGACGATGATGAGGGCTCGATCCGCGCGTC13200.16220022142787804No Hit
GATGAGGGCTCGATCCGCGCGTCGTTCAAACAATTCGTGGTGGCACTTGG13140.16146294769411496No Hit
GATTTCGTCGATGAGGAAGCAGTCAAATTCGATCGCCATCGATAACGCGA13070.16060279500472469No Hit
CGCTCACGCGCAATCTCGCGATTGAACTCGCCGATGCGGGTATCCGGGTG12950.15912824753719854No Hit
GTACAAGAGTTCTCGGAACTGGGCTACTACCTGCGAGAGCCGGTCAAGAG12900.15851385275906263No Hit
TTCCTGCGTCGCGCGGCGCGCGTCGCGCCCGTGCTGATCCTCTGCCTCGC12870.15814521589218108No Hit
TGTCAGGGGCATCGGTCGGGTGGAATGAGCGCGTCAGTTGATGGCGCTGT12780.15703930529153645No Hit
TCTCGGAACTGGGCTACTACCTGCGAGAGCCGGTCAAGAGCTATTCGGCA12430.15273854184458516No Hit
TCCGCGAACATTGCACTCGGGCAGCCGTGCTGGTGCAAGGCCGGTTGCGC12260.15064959959892307No Hit
AGCAAAAGCAGGGCGAGCGAGCGCACCACCCGCTGAATGCCGGTGCCTGC11610.14266246748315636No Hit
GTCAAATTCGATCGCCATCGATAACGCGAACGCGAGGCGCGCACGCATAC11600.1425395885275292No Hit
GCCTTGCACCAGCACGGCTGCCCGAGTGCAATGTTCGCGGATGTACGCGG11360.13959049359247686No Hit
GACTACTACACGCGACAGGGCCGACGCAGGGTCCTCGAAAAGATCGACCT11210.13774730925806916No Hit
TCATACGGCATCGCCGCGCGACATTTGTCGTGGAATAGATGCCCGGGATC11170.13725579343556044No Hit
GTCTTCCTTCGCCATGAAGCCTTCATAAATCGACGTCTGTACGATCGCCG11140.1368871565686789No Hit
GGGCGAGCGAGCGCACCACCCGCTGAATGCCGGTGCCTGCATCATGTTGC11100.13639564074617017No Hit
ATCGTACAGACGTCGATTTATGAAGGCTTCATGGCGAAGGAAGACATTGC11010.13528973014552556No Hit
CGAAAGCAGGTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCGCC10970.13479821432301684No Hit
CTCTTGTACGAACGGTTCGGCATCTTTGACATTGACGCCATAGATGCGGC10930.13430669850050808No Hit
GAATGAGCGCGTCAGTTGATGGCGCTGTTTTCCTGGTAGAACGCGTAGGC10920.13418381954488093No Hit
ATTCGTGGTGGCACTTGGCGTGAAACCGGCTGTCGCCGACCGCAATGATT10770.1323406352104732No Hit
GGTGGAATGAGCGCGTCAGTTGATGGCGCTGTTTTCCTGGTAGAACGCGT10450.12840850863040346No Hit
ATGCCGAACCGTTCGTACAAGAGTTCTCGGAACTGGGCTACTACCTGCGA10430.1281627507191491No Hit
AGCGAAAGCAGGGCAAGGACCGCTGACATGGCGCCGTGCCCTCTTTCCGA10400.12779411385226755No Hit
GTTGACGCGCAGGTCGATCTTTTCGAGGACCCTGCGTCGGCCCTGTCGCG10300.12656532429599576No Hit
ACGATGATGAGGGCTCGATCCGCGCGTCGTTCAAACAATTCGTGGTGGCA10170.1249678978728424No Hit
ATCATCGCCTGGTGCGCGAGGGTCATCACTTGTTCGGGCGTCATCGTGCG10120.12435350309470651No Hit
TAGTAGTCTTTGGCGATGTTCTTGAGTTCGATCATCCGAATTCAACTATT10120.12435350309470651No Hit
CATCAGGCTCATCAGCGGTGCGGAACTCCCGAGTGCCGGCATGGTTCATC10040.12337047144968906No Hit
CCTATTCGCTGAGCGTGCAGACGATGCTGCTGCTCACGATGCTGTCGTTC10030.12324759249406186No Hit
CGATAACGCGAACGCGAGGCGCGCACGCATACCTGCCGAATAGCTCTTGA10020.1231247135384347No Hit
GTTCATACGGCATCGCCGCGCGACATTTGTCGTGGAATAGATGCCCGGGA9930.12201880293779008No Hit
GCGATGAACCATGCCGGCACTCGGGAGTTCCGCACCGCTGATGAGCCTGA9700.11919258695836492No Hit
GGCTCGATCCGCGCGTCGTTCAAACAATTCGTGGTGGCACTTGGCGTGAA9690.11906970800273774No Hit
GTGCTCGACCGCGCGTACAACGACGGCTACAAGCCGTTCTCCGACGGCTC9620.11820955531334748No Hit
GGGCAAGGACCGCTGACATGGCGCCGTGCCCTCTTTCCGACCCCGCATTC9600.11796379740209313No Hit
GTTCAAACAATTCGTGGTGGCACTTGGCGTGAAACCGGCTGTCGCCGACC9510.11685788680144849No Hit
TGCCGGCACCGTTCCTGCCGAGAATGCCGATTTTCTCGCCCCTGTTGACG9470.11636637097893977No Hit
TTTCCTGGTAGAACGCGTAGGCCTCTTCGATCTCGTCAAACATGCGCAAC9390.11538333933392234No Hit
CATTCCACCCGACCGATGCCCCTGACAAGGTTCAATCGCGCCGCACTCGC9360.11501470246704079No Hit
CCCCCGACGTTGCAGTCGAACCCAAGCGCCTGAAGTCGATTTTCGAAGTA9330.11464606560015925No Hit
CATCATCGTCAGCCACGATGCCGCGTACATCCGCGAACATTGCACTCGGG9320.11452318664453207No Hit
GATCGAGCCCTCATCATCGTCAGCCACGATGCCGCGTACATCCGCGAACA9260.113785912910769No Hit
GCGAAAGCAGGAAGACAATCGTGTTCGCAACGTCTTCGGGCGTACCGACG9130.11218848648761565No Hit
TCATTGCGGTCGGCGACAGCCGGTTTCACGCCAAGTGCCACCACGAATTG8960.11009954424195358No Hit
GAATTTGACTGCTTCCTCATCGACGAAATCATTGCGGTCGGCGACAGCCG8950.1099766652863264No Hit
GAACTGGGCTACTACCTGCGAGAGCCGGTCAAGAGCTATTCGGCAGGTAT8930.10973090737507205No Hit
GGGCTACTACCTGCGAGAGCCGGTCAAGAGCTATTCGGCAGGTATGCGTG8930.10973090737507205No Hit
CTCATGCTGCGATGAACCATGCCGGCACTCGGGAGTTCCGCACCGCTGAT8910.10948514946381768No Hit
GTCAAGAGCTATTCGGCAGGTATGCGTGCGCGCCTCGCGTTCGCGTTATC8900.1093622705081905No Hit
ACCTGCGGTTCATCTGCCGCATCTATGGCGTCAATGTCAAAGATGCCGAA8870.10899363364130896No Hit
CTTCGATCTCGTCAAACATGCGCAACCGGCCTTGCACCAGCACGGCTGCC8860.10887075468568178No Hit
TAGTAGCCCAGTTCCGAGAACTCTTGTACGAACGGTTCGGCATCTTTGAC8780.10788772304066434No Hit
ATTCGGCAGGTATGCGTGCGCGCCTCGCGTTCGCGTTATCGATGGCGATC8750.1075190861737828No Hit
CTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCGCCGATGCGGGTAT8730.10727332826252843No Hit
CAATTCGTGGTGGCACTTGGCGTGAAACCGGCTGTCGCCGACCGCAATGA8710.10702757035127408No Hit
CAATGATTTCGTCGATGAGGAAGCAGTCAAATTCGATCGCCATCGATAAC8710.10702757035127408No Hit
ATGATGATGCGCGTGAAGCTCGTCATCATCAGCAGCATCGCCGGCAGGAA8690.1067818124400197No Hit
GTCAAAGATGCCGAACCGTTCGTACAAGAGTTCTCGGAACTGGGCTACTA8520.10469287019435765No Hit
CTTTCATCGCACCTGCAATGTCTTCCTTCGCCATGAAGCCTTCATAAATC8510.10456999123873045No Hit
CTACTACACGCGACAGGGCCGACGCAGGGTCCTCGAAAAGATCGACCTGC8470.10407847541622174No Hit
CTACCTGCGAGAGCCGGTCAAGAGCTATTCGGCAGGTATGCGTGCGCGCC8460.10395559646059456No Hit
GGTATCCGGGTGAACGCTGTCTCGCCGGCGATCGTACAGACGTCGATTTA8450.10383271750496739No Hit
CATCAGCGGTGCGGAACTCCCGAGTGCCGGCATGGTTCATCGCAGCATGA8400.10321832272683147No Hit
GAGCAAAAGCAGGGCGAGCGAGCGCACCACCCGCTGAATGCCGGTGCCTG8300.10198953317055968No Hit
GTTTTCCTGGTAGAACGCGTAGGCCTCTTCGATCTCGTCAAACATGCGCA8280.10174377525930531No Hit
ATTGAACTCGCCGATGCGGGTATCCGGGTGAACGCTGTCTCGCCGGCGAT8250.10137513839242376No Hit
TGTCGTGGCCGCTCGCATTCGGCGGTGCGTTCCAGGGCAGCCTCACCGGG8190.1006378646586607No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA24600.0131.170381
GCGAAAG28500.0112.9663242
GAGCAAA3050.0106.985161
CGAAAGC31550.0102.0456543
GAGCGAA2950.095.863541
GAAAGCA35850.089.805864
ATAGGGA259.379859E-487.01464
AGCAAAA6450.078.695371
AAAGCAG50850.073.296475
AGAAACA502.3035554E-672.512174
AGTAGAA502.3035554E-672.512171
CACTGTG300.001935739172.4988145
TACACAA608.030656E-872.4988145
AGCAGGA11050.070.202467
GGTATGG554.061323E-665.908145
CCTATCA2100.062.1532862
TCTCTTT350.003566498262.14183145
AGAGGTG350.003566498262.14183145
AAAAGCA8200.061.0163424
GCAAAAG8200.061.0163422
Produced by FastQC (version 0.11.5)