FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2383753
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA49560.20790744678664272No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT34320.14397464838009644No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG34110.1430936846225259No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA33250.13948592828199902No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT33100.13885666845516292No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT32490.1362976784926962No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA30020.12593586667746196No Hit
ATTCTACACTGTAGAGACCCATTAGAACACATCCAGAAACTGATTGCCCC29980.12576806405697236No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT29270.12278956754328155No Hit
GCAGAGGCCATGGAGGTTGCTAATCAGACTAGGCAGATGGTACATGCAAT24830.1041634766689334No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT24800.10403762470356619No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG24510.10282105570501641No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA24080.10101717753475296No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT23970.10055572032840651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9600.0108.779671
CGAAAGC28950.077.4029163
GCGAAAG29800.075.680222
GAGCAAA62250.073.859511
AGCGAAA33600.066.9081341
CAAAAGC94350.049.037264
AAGCAGG107950.048.0891047
AGTAGAA32850.047.6842461
GCAAAAG98750.047.0726173
AGCAAAA101250.045.4796372
GTAGAAA41150.045.291821
TAGAAAC41450.044.7871782
CAATTCG9050.043.261499
AAAAGCA111350.041.942295
GCAGGGT25950.041.6298839
GAAAGCA56150.040.8117034
AAAGCAG143900.039.258456
GCAGGTA20850.035.8168569
ACAAGGG37700.035.0088163
AGCAGGT43600.034.0890248
Produced by FastQC (version 0.11.5)