FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3652747
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTATAGTGATTCGTTTCCCAAGATAAAAAGCCGCTATGTAAGCATCT74220.20318954474536563No Hit
GTATCAGTACACGGCACAGGCAGAAAACAGCCTGCAAATCACCCTACCGA66190.1812060895539713No Hit
GTATATGTGTATTATTACGGCAATGCTAAACGACACAAAGAGGCTCTTAC45520.12461854051211321No Hit
CACATATACTTTGCCGTCTTTTTCTGTTGAAATAGGCGAGAGCCCTTTGA44950.12305807109005908No Hit
CTATAGAGAAAGCTAAACAAATAGAAAAATAGAACCTATAAATAAAAACT44910.12294856446395No Hit
CTATAATACTCATTCTTGCAGTCGTGAATACACAGGCACAGGAACGACAA41980.11492720410146116No Hit
GTATACTTATCGAGACGGCCTTTCTTACTAGTAACGAAGAGCGAAAGTTC40180.10999940592655337No Hit
ATCTTACAAAGTGCAAATTTCGTCTAGCCCCAAACGTTTGGAAGCTAAGC39730.10876745638282641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG81100.043.1850852
CGAAAGC82950.042.0476883
AGCGAAA88600.039.2042471
GAAAGCA102700.035.3737224
AAGCAGG128500.031.8274126
GAGCAAA74200.031.7622131
AGCAAAA91650.025.3177452
AGCAGGG61050.023.9890887
AAAGCAG202900.023.9451795
AGCAGGC49450.023.4585827
TCTATAG68900.022.4168113
CAAAAGC105450.022.0735364
TATAGTG65800.021.8204965
TATTATA74900.020.8144132
CTATAGT67850.020.5194074
GTACACG50250.019.910917
GCAAAAG125000.019.6074073
TACACGG52650.019.003558
ATATGTG45800.018.8406093
TAACGTG15250.018.5447145
Produced by FastQC (version 0.11.5)