FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1792916
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG26420.14735771224084118No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATAACACTGAAATTCGCTT26420.14735771224084118No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA25520.14233795671409036No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC25390.1416128809157819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA13250.080.995971
GAGCAAA64000.073.419711
GCAAAAG70850.064.8829653
CAAAAGC74200.062.539934
CGAAAGC21550.062.2451823
AGCGAAA22050.060.18111
AGCAAAA77650.059.1076242
GCGAAAG23500.057.0801542
CGAGTCG553.219882E-452.726643145
AAAAGCA95950.048.5144775
AAGCAGG113700.048.4602857
AGTAGAA23950.045.718191
AAAGCAG125600.045.374746
AGCAGGG58250.040.5746238
TAGAAAC31700.038.197812
AGCAGGC23200.033.74968
AGGCTCG1751.0100666E-733.142464145
GCAGGGT30950.032.3259479
CTAGGCG950.0047285630.5259519
CATACCG2401.5843398E-930.2079729
Produced by FastQC (version 0.11.5)