FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4637640
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCG231080.4982706721522154No Hit
GAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCGTGGTC147240.31748906771547597No Hit
TACTAGGGACGGCCCTTCTCAAATTTCCTGCGCGCACAGAGGATAGGGAC139340.3004545415340561No Hit
CTAGTGATCCGGCCATGGCACGTGGGTGCGTGGTCGCTCAACGGATAAAA97670.21060280659990857No Hit
TAGCCAACCAGCCATGCACCTGGCGGTACAACTGGCACACCAGAGGTTCG90440.19501298074020407No Hit
GATAAAAGGTACCCCGGGGATAACAGGCTGATCTTGCCCAAGAGTCCATA65920.14214126150369583No Hit
GAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCG65790.14186094651590034No Hit
GGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACG58670.12650831026125356No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA56950.1227995273458052No Hit
GCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCGAA55320.1192848086526768No Hit
AAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCGAACGT50590.10908565563519376No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG49850.1074900164738962No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT48020.10354404395339009No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC46530.10033120293942609No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA180900.094.402591
AGCAAAA202450.082.9891052
GCAAAAG217050.077.709143
AAAAGCA236850.071.4585655
CAAAAGC238450.071.039144
CGTCGAC2050.067.196557
GAGCGAA58800.066.595981
ATTTCCG11450.063.951946145
AAGCAGG328700.061.186277
AGCAGGC107950.059.572188
GCAGGCT87600.056.941239
AAAGCAG367200.056.3214346
GCGAAAG74700.053.5835272
AGCGAAA75850.052.199771
AGTAGAA49100.051.543691
CGAAAGC80100.050.153293
AGCAGGG116950.042.775148
GTAGAAA87600.038.4928781
AGCAGGA152650.037.663358
CTAGGCG3600.036.2503789
Produced by FastQC (version 0.11.5)