FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6107061
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG63700.078.8887942
AGCGAAA63750.077.5801541
CGAAAGC67600.073.801783
GAGCGAA29350.062.511351
GAGCAAA148100.052.882881
GCAAAAG183650.041.972753
CAAAAGC184600.041.678184
AGCAAAA208900.036.6214522
AAAAGCA247750.031.522975
AAAGCAG304900.031.132026
AAGCAGG296450.030.0074657
GAAAGCA192600.026.9576384
TACGACG7250.020.9999127
ACGACGC7350.020.7143698
AGCAGGG205650.020.4474438
CGACGCT7450.020.4363259
GTATAGG24850.020.135811
TATACCG3252.2021644E-517.8497335
CACGAGC17200.017.281904145
CTTTCGC14150.016.3989122
Produced by FastQC (version 0.11.5)