FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7671657
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG137810.17963524698771074No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA111420.14523589884167137No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA111020.1447144990971312No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC94420.12307640969871306No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC92520.12059976091214715No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT89140.11619393307078249No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA88630.11552914839649375No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG82270.10723889245830465No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG82210.10716068249662361No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT80980.10555737828216252No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT80470.10489259360787376No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA77060.10044766078566861No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG94300.083.8174062
GAGCGAA35400.079.480251
AGCGAAA99400.078.935361
CGAAAGC100400.078.654223
GAGCAAA240950.063.893071
CAAAAGC295050.051.734994
GCAAAAG332700.045.8368033
AAGCAGG390100.044.6988377
AGCAAAA355500.043.039012
AAAAGCA368700.042.0111435
CCGCACG5600.041.427963145
AAAGCAG444100.040.8877076
GAAAGCA200700.040.069334
GGTCGTT32500.038.3709227
AGTAGAA104050.034.4283031
CAATTCG62750.033.85439
AGCAGGT169100.033.4863178
TAGAAAC113150.033.3889352
GTAGAAA124500.031.9184591
GCAGGTC78900.031.335559
Produced by FastQC (version 0.11.5)