FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6999373
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG85050.090.7087552
CGAAAGC91750.083.929133
AGCGAAA92550.082.655231
GAGCGAA26800.077.108871
GAGCAAA138650.049.211211
GAAAGCA198300.039.5638774
CAAAAGC205750.033.3384634
AAGCAGG325900.032.6394736
GCAAAAG208500.032.6205333
AAAGCAG361900.031.7769345
AGCAAAA222550.030.3973642
CTCGATA25900.029.3925519
TAGACCG2802.6375346E-1028.4860385
AGCAGGG214200.026.5025047
AAAAGCA270800.025.5444835
CCTCGAT33300.022.643158
TATATCG6350.021.6958795
CAATTCG6050.021.570739
GACCGTA4501.3278623E-1020.9447657
GGCCTCG38000.020.0356336
Produced by FastQC (version 0.11.5)