FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6157095
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAAGTGACTGAGTCGGAGATAGCACAAGGTATTGACCAACAGATA64610.1049358504294639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC102100.076.5584953
AGCGAAA105300.073.4055941
GCGAAAG151700.051.5255852
GAGCAAA149400.045.8164831
GCAAAAG167400.040.5434533
GAAAGCA227000.035.328864
GAGCGAA67600.035.289831
AGCAAAA195750.034.8930472
CAAAAGC198250.034.1978454
AAGCAGG298600.033.8514146
AAAGCAG407200.028.972115
AGCAGGG175150.025.8710337
GTATAGA55500.025.215331
AAAAGCA293400.023.9229975
TCTATAC50350.023.4740753
TAGGACC11650.022.4066494
GTGCGTT18350.019.7553589
TACTATC48700.019.502327
GCAGGGG80950.018.4500628
GTGACTG59900.018.1558029
Produced by FastQC (version 0.11.5)