FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4875604
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA91210.1870742578765626No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC57780.11850839403692343No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT57370.1176674725839096No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG54120.11100163179782443No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC52710.10810968241063057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA30150.088.989111
AGCGAAA52200.065.846131
GAGCAAA168950.062.4921721
CGAAAGC58750.060.1064763
GCAAAAG175750.059.5760963
GCGAAAG62850.055.8393362
CAAAAGC190150.055.2169464
AGCAAAA189750.054.7601472
AAGCAGG262200.048.525877
AAAAGCA235750.044.5981145
AAAGCAG321150.040.4834026
AGCAGGG137150.038.5887268
AGGCTCG5850.034.70084145
CAGTACG7350.034.5237969
AGTAGAA52250.033.7242621
CGCGAAA1204.055709E-430.2142071
TAGAAAC69750.029.108112
GCAGGGT78500.028.630569
GCAGTCG3403.6379788E-1227.7248426
GCAGGTA80700.027.6703729
Produced by FastQC (version 0.11.5)