FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8416570
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG161660.19207349312130714No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA124290.14767298317485625No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA115430.13714612959911224No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT95190.11309832865407166No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT86410.10266652567494834No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA40350.090.03791
GAGCAAA228950.072.9748151
AGCGAAA75350.072.563681
GCGAAAG79000.070.0350952
CGAAAGC83800.065.8524253
AGCAAAA325900.050.552462
CAAAAGC338200.049.036984
AAGCAGG403250.048.545147
GCAAAAG366600.045.317283
AAAGCAG481800.041.661826
AAAAGCA420150.039.749215
GCGAAGC8000.038.977113
AGCAGGG240200.038.877588
GTAGAAA109200.036.789031
TAGAAAC117250.034.3858832
CAATTCG21850.034.178469
TCGTGTA10850.032.7489624
CGTGTAG10700.032.5309265
GCAGGTA71750.030.5176969
AGTAGAA100600.027.5357281
Produced by FastQC (version 0.11.5)