FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2653335
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA21750.091.028961
GAGCAAA77950.082.059461
AGCGAAA41850.076.0755841
GCGAAAG49450.066.14092
CAAAAGC102950.062.202894
CGAAAGC52950.062.0452353
AGCAAAA102350.061.7149122
GCAAAAG110400.057.6768653
AAAAGCA125800.051.1927535
AAAGCAG179700.045.9272466
AAGCAGG185050.043.803237
AGTAGAA44650.031.3481581
AGCAGGC55650.031.1370858
GAAAGCA108800.030.9956134
TAGAAAC47000.029.9350553
GCGAAGC5050.028.7220083
AGCGTCA4300.028.6719133
AGCAGGG101800.027.6330748
ATCGTGT8300.027.0869333
TATCGTG9000.026.590792
Produced by FastQC (version 0.11.5)