FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7803636
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA283700.36354847919610805No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG240020.3075745716483957No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT219770.2816251296190648No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC212570.2723986613419693No Hit
CATTTCACACCATCGATTTCTTTTACGATAACTTCTGGACGAGATACTTG181480.23255825873990024No Hit
CTCCAACAGACGCGGTTGAAGCTCTTCCAATCCTACACATCGATGAGCCA161250.20663444578911677No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC158410.2029951166353736No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA143190.18349138786073568No Hit
GGTCAAAGGTATGGTTCATGATACCGTAACCACGAGTCATTGACAAGAAC135520.17366263623777428No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC130040.16664026871576273No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC128910.16519222577782974No Hit
CTATCACTCCGACAGATGCAGTTGAAGCTCTTCCAATCCTACACATCGAT127310.16314189949403074No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC126410.1619885909593938No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA122520.15700373518190752No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA116490.14927656799984007No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA113780.14580382785665552No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT105130.13471925138486726No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT104220.1335531283109566No Hit
GAGCAAAAGCAGGGAGCTTGAAAAGGAGATGGTATTGATTGCAAACAGGA102780.1317078346555375No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG100570.12887582147604015No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG96370.12349371498106779No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT95490.12236603552497835No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC90170.11554870063134673No Hit
GGTCAATAACTCACCATTTGCTGGTAAAGAAGGTAAATGGGTAACTTCTC89950.11526678076732436No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT87760.1124603966663745No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT86130.11037162676475429No Hit
CAGTAGAAGCTCTTCCAATCCTACACATCGATGAGCCAACTCTTCAAATG85740.10987185973307828No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG85010.10893639836609499No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTT82070.10516892381961433No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG81020.10382339719587128No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA80420.10305452483944663No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA47300.096.584421
GCAAAAG277350.084.02893
AGACGCG31950.083.7357258
GACGCGG32550.081.969999
GAGCAAA310800.076.038061
AGCAAAA324150.072.052212
CAAAAGC329400.071.3894964
CGAGTCG3100.070.16094145
GGGTCGC6100.068.934074145
GCAGGGT102600.063.8114289
GCGAAAG79800.061.516042
CGAAAGC80900.060.6807753
AGCGAAA82400.059.0504151
AAAAGCA405050.058.1823465
CAGACGC47900.057.8203057
AAGCAGG488050.056.109347
AGCAGGG219500.054.9304358
ACCATCG58100.054.1591269
TAGAAAC125250.053.0298422
TACGTTA2650.051.98087145
Produced by FastQC (version 0.11.5)