FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53201
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT1230.23119866167929173No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC980.18420706377699667No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT960.18044773594481306No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC920.17292908028044587No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC870.16353076069998684No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA740.13909512979079341No Hit
CTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAATGTTATTT650.12217815454596719No Hit
TTCCCATTGAGGGCATTTTGGACAAAGCGTCTACGCTGCAGTCCTCGCTC630.11841882671378358No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG630.11841882671378358No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG630.11841882671378358No Hit
TCCTAGTTCCAGTACTGGTTTAAGAGATGATCTTCTTGAAAATTTGCAGA590.11090017104941635No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT570.10714084321723277No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA570.10714084321723277No Hit
TTCCAGTACTGGTTTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC570.10714084321723277No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATA560.10526117930114096No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG550.10338151538504915No Hit
GGTTTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT540.10150185146895735No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA203.8319628E-4108.8480761
CGAAAGC259.3041954E-487.078463
AGTAGAA406.575924E-572.5653841
TCTATGC553.9875904E-665.968533
CTATGCT553.9875904E-665.968534
GCAAAAG451.1782589E-464.502563
AGGGTGT350.00353530962.19896
GCAGGGT350.00354852162.140448
TATGCTG651.0737942E-555.8195235
GCGAAAG400.005998108554.4240382
AAGGGTG400.005998108554.4240385
TAGAAAC604.8809114E-448.3769233
ACATGGG604.8809114E-448.3769236
AATCAAA450.00955529248.3769231
ACAAGGG450.00955529248.3769233
CTCTATG803.6672664E-545.3533632
CATGGGT657.27528E-444.6136477
TTCCAAC1002.668763E-643.539231
GCTGACA855.274551E-542.64548
TCCAACA1053.7328355E-641.465932