FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7215116
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA234500.086.059711
GAGCGAA63200.067.581481
GCGAAAG100600.064.655112
AGCGAAA100900.063.3879781
GCAAAAG315350.063.3503763
AGCAAAA315500.063.180612
CAAAAGC323750.061.773894
CGAAAGC121650.053.4688453
AAAAGCA378150.053.2139325
AAAGCAG497550.048.780416
AAGCAGG461050.048.7174577
AGCAGGG238050.038.5581978
AGCAGGC144250.031.5139568
CGATTAC31650.026.8065931
GCAGGGG125900.026.3750929
CGGTCAC15250.025.677365
GAAAGCA265100.025.4933364
CCCTGTA67550.024.5790089
AGTAGAA93550.024.2621921
TACGGGC15650.024.0940324
Produced by FastQC (version 0.11.5)