FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10027617
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA287000.078.351
GCGAAAG113200.069.5616762
AGCGAAA120450.064.416341
CGAAAGC140050.056.5898933
AGCAAAA394150.056.0896262
GCAAAAG401300.055.14643
CAAAAGC415000.053.3783234
GAGCGAA79250.050.9653551
AAAAGCA493500.045.137335
AAGCAGG572850.042.9048547
AAAGCAG628750.041.4363026
AGCAGGG293800.032.919228
AGCAGGC181850.027.7486218
GAAAGCA318800.025.633474
AGTAGAA127450.023.4410211
GCTAGCC34600.023.2620873
AGCAGGT204100.021.8462858
GCAGGGG151350.021.4126599
AACAAGG219450.021.0800552
GTAGAAA167350.020.9719491
Produced by FastQC (version 0.11.5)