FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9802733
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG474070.48361002997837443No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA330950.33760992980222965No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA299720.30575146747340765No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA231410.236066819324774No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT220650.22509028859604763No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT213910.21821465503548856No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT210090.21431778260205597No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC207300.21147163755250703No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAACGGTGAAATTTCTCCAACT191890.1957515317411991No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC178600.1821940881180789No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG175620.179154119570532No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG172230.175695900316779No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT168570.17196224767113416No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC152410.15547704910457116No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT150170.1531919720755426No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA149450.15245748303049772No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAGAAAAGAATAGAGA149180.15218204963860588No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC144870.1477853166050733No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCGT138080.14085867686083056No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC137400.1401649927627326No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT135880.13861440477874895No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG132830.1355030275740449No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA131400.13404425072069187No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT122560.12502635744541854No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGG119740.12214960868565941No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT117900.12027258112610023No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC116540.11888521292990435No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT115100.11741623483981457No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG108670.11085683961809426No Hit
AGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACC108610.11079563219767384No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGT107020.10917363555653307No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT104920.10703137584181881No Hit
AGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTG104110.1062050756661433No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG103700.1057868249599372No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC103250.10532776930678414No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC102290.10434845058005762No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC101040.10307329598796582No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC99940.10195115994692501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA36600.0127.0012361
AGGCTCG8650.080.46159145
GCAGGGT171250.077.47729
GCAGGTA91200.076.63659
GAGCAAA327350.065.7258451
GCGAAAG82800.064.804592
GTAGAAA117000.064.024381
CGAAAGC84350.063.5290833
AAGCAGG415200.063.1597187
AGTAGAA87350.063.0930671
AGCAGGG249450.062.8087658
GACAGTA20850.055.2891737
TAGAAAC143600.052.2625162
CGGCGGC4400.051.079018145
GCAAAAG436750.049.5592423
GCTAGGT2500.049.299488145
AGCAAAA448250.048.189742
CAAAAGC455000.047.698924
GGATTAA6100.047.54049145
CAGGTAC25400.047.1046143
Produced by FastQC (version 0.11.5)