FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7816676
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCAAAGGTATGGTTCATGATACCGTAACCACGAGTCATTGACAAGAAC92860.11879729951708373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA39500.097.665341
AGCGAAA103750.077.092711
GCGAAAG107800.074.9315262
GAGCAAA231550.073.094041
CGAAAGC111850.072.347973
CAAAAGC295150.056.897594
AGCAAAA300000.055.905242
GCAAAAG307150.054.7690963
AAAAGCA369650.045.8624765
AAGCAGG458050.043.083357
AAAGCAG481300.042.8467036
GCGCGAA1903.6379788E-1241.9821851
GAAAGCA262850.031.5033154
AGCAGGG279550.029.824468
CGCGAAA2451.9736035E-929.5959342
GACGCAG37950.028.6562449
AGCAGGC157250.025.4958048
TCGCTCC22700.025.2350945
CAGACGC44600.024.3831987
TTCGCTC23800.023.7637944
Produced by FastQC (version 0.11.5)