FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7348140
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCG149810.20387472203850227No Hit
TACTAGGGACGGCCCTTCTCAAATTTCCTGCGCGCACAGAGGATAGGGAC111480.1517118617772661No Hit
GTATTTAAATGGTCTGTTTGGTAGCGAACAAGGCAAGAGATTCGTTAAAT106640.14512516092507763No Hit
GAGCAAAAGCAGGCAGCGGAAGGTATTGGATGTGTTCAAAAGATACCAAG95600.13010095071678002No Hit
GTATTGGATGTGTTCAAAAGATACCAAGAAGAATATGACAGGTATTTAAA91760.12487513847041563No Hit
GAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCGTGGTC90520.12318763659919382No Hit
GAGCAAAAGCAGGGATAACAAGCAAGTCCCCTCCGTCATCCCTGCTGATT87780.1194588018192359No Hit
TCGTAAAGGTGGTGCAGGCGGTTGGGAACAGCTTTGATGACCGGCCTTAA85500.11635597579795703No Hit
TTGTAGTAGTGGGTGGCAACCTGGTATTCGTGTGGCTCCCATTCATGATA84320.11475012724308466No Hit
CTCCAAGCCCGCGTAGCCGTGCAGTACGTCGAATTTTGCGCACAGGTAAT83130.11313066980215403No Hit
GTAGTAGTGGGTGGCAACCTGGTATTCGTGTGGCTCCCATTCATGATACC75970.10338670738445377No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA253100.078.0109251
GCAAAAG271450.072.70923
AGCAAAA275700.071.245012
CAAAAGC324200.060.878814
GCGAAAG118000.058.3131372
AAGCAGG408950.057.4405487
AGCAGGC162900.050.159088
AAAGCAG514750.048.1332976
GCAGGCT83350.046.973159
CGAAAGC156350.044.2890663
AAAAGCA456350.044.25055
AGCAGGG175700.038.9948048
GCAGGGA106800.038.017049
GAAAGCA203000.034.718534
AGCGAAA201000.033.621711
GAGCGAA146100.033.3021471
AGCAGGT160200.027.878218
GTTAGGT16900.026.5990739
AGCAGGA168800.026.1142678
TGCGTAC19700.024.6603744