FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8471308
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG85050.082.357472
CGAAAGC86500.080.977873
AGCGAAA86450.080.191141
GAGCAAA188700.059.027291
GAGCGAA37450.051.5066531
CAAAAGC258150.042.9477734
GCAAAAG261900.042.332833
AGCAAAA286550.038.766672
ACGGACG10950.035.090755145
AAAAGCA355550.031.8148025
AAGCAGG392300.031.4910667
AAAGCAG423200.030.7213236
GAAAGCA241450.030.2417934
AGCAGGG262250.022.1438487
ATGCCGG31000.021.51578145
CTCCGAC12750.020.470257145
AGCAGGC135200.019.5728848
TACCCCG11050.017.0614685
CACGAGC39550.016.864454145
AGACGGC75850.015.962168145
Produced by FastQC (version 0.11.5)