FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8257364
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT196340.2377756388116111No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT181170.2194041585183843No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC167260.20255858891530032No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC149660.18124428086251254No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG141550.17142274459500637No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA141280.1710957637328329No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT124220.150435417404392No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA116070.14056543952767495No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC107280.12992039590358376No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA105890.12823704998350563No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG100790.12206074480911824No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAGAAAAGACG95030.11508515308275136No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA94840.11485505543899967No Hit
GTACAAATTGCTTCAAATGAGAACATGGATAATATGGGATCGAGCACTCT94330.11423742492156093No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA92320.11180323405871413No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC91600.11093128509291827No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT90730.1098776800925816No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT88670.10738293721822123No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG87740.10625666980406821No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC86270.10447644066556834No Hit
TTCTTAATCTGTGTCTCACTTCTTCAATTAGCCATCTTATCTCTTCAAAC86210.104403778251752No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT83280.10085543037705495No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA82680.10012880623889173No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA75550.082.159361
CGAAAGC81700.076.9510963
GAGCGAA34900.076.668811
GCGAAAG92700.067.816612
GCAAAAG218700.055.9350553
CAAAAGC258750.048.0619664
GAGCAAA259600.047.9325331
AGTAGAA76700.045.2853741
TAGAAAC112000.043.182182
AGCAAAA286650.042.774842
AGGCTCG8200.038.017906145
AAGCAGG381800.037.9986767
AGCGTCA6950.037.5608563
AAAAGCA333050.037.3833855
GCAGGGT94650.036.0792589
AGGGTGT85150.035.682056
CAAGGGT107150.035.5291564
CGGTCGA1301.6563446E-533.4612145
ACAAGGG116000.033.1935963
AGCAGGG221600.032.4890338
Produced by FastQC (version 0.11.5)