FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8670085
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA138600.15986002444036015No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT115970.13375878091160584No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA104060.12002189136554026No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA103310.11915684794324394No Hit
ATTCTACACTGTAGAGACCCATTAGAACACATCCAGAAACTGATTGCCCC102700.11845327929310959No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT88180.10170603863745281No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG107250.078.563492
GAGCGAA35250.076.531171
AGCGAAA113750.073.1251141
CGAAAGC115700.073.0145653
GAGCAAA201650.065.200971
AGCAAAA306550.042.8143352
CAAAAGC324700.040.6895644
GCAAAAG328450.040.2470783
TAGAAAC118050.038.8820952
GAAAGCA239250.035.945934
GTAGAAA131150.035.7758941
AAGCAGG426000.035.093237
AGTAGAA124600.033.2332341
AAAAGCA414250.031.946065
AAAGCAG526450.030.2616356
CTACACT66750.029.5484434
AGCAGGG219950.028.9082938
CTATACT43350.028.6038154
GGGTGTT113600.025.145617
GGTGTTT113450.023.3257268
Produced by FastQC (version 0.11.5)