FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6882608
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG263620.38302341205543017No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA173950.2527384968023749No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA136860.19884904094494413No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA127660.1854820149571209No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG122640.178188268168113No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT110040.1598812543152247No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC95940.13939483405127823No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT91170.13246432166411337No Hit
GTACTATGTTCTTTTCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGG90870.13202844038190173No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC90460.1314327359628792No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT89310.1297618577144013No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT87720.12745168691867967No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC85300.1239355779088392No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT83270.12098611456587388No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC82690.12014341075359805No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT82320.11960582383887038No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA79720.11582818605970295No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT77700.11289325209281134No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG75680.1099583181259197No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT72470.1052943884062553No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA32550.0113.6117861
GAGCAAA233550.068.925091
GCAGGTA63250.064.53649
GCGAAAG64050.062.716112
AGGCTCG6500.062.461082145
CGAAAGC66350.060.4345553
AAGCAGG336500.058.303757
GCAGGGT134650.057.560999
AGCAGGG201600.053.5501988
GCAAAAG306050.052.7392353
AGCAAAA305350.052.5261572
GCGAAGC4150.050.6700173
GTAGAAA74800.050.5056951
CAAAAGC327750.049.4465374
AGTAGAA59700.047.854871
AAAGCAG443100.044.6420026
TAGAAAC87800.042.7783162
AGCGAAA95350.041.7499431
CAATTCG11250.040.601779
AGCAGGT141550.040.3620538
Produced by FastQC (version 0.11.5)