FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGAGTGGTCAGGATATAGCGGGAGTTTTGTTCAGCATCCAGAACTAAC11.9607843137254901No Hit
GAGCAAAAGCAGGGCACAGTTAGTAAACTCATACTTTTTGTTATGATTGA11.9607843137254901No Hit
CAGTGGTGTGATCATAGCTCACTGTAGCCTTGAACTCCTGGGCTCAAGTG11.9607843137254901No Hit
ATTCAACAGTACTTAAATTCTCTCAGCTTCTGGAGTCACTTAGTCTATGT11.9607843137254901No Hit
ACAATATCAAGTGCAAGATCCCAATGATATTCGCGGCACCAACAAGGGGG11.9607843137254901No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT11.9607843137254901No Hit
ACTATAGATATATGTTGCCACAAGGACCATAGTCAAAATAACCAGCACTC11.9607843137254901No Hit
CTCTTGTTGGGAAACAAATCGTGGAATGGATCTTGAATGATGAATCCAGC11.9607843137254901No Hit
GTTGGGATGCGACTCCTCTGCTTTTCATAGACAAACCAGAAACATGAAAT11.9607843137254901No Hit
ACCCTCCCCAGCTAAGAAACTAAGCCACAAGATAGCATCAGTCATACATA11.9607843137254901No Hit
TTTGACATCCTCAATAGTATGTCCTGGAATAGAAGGCAATGGTGAGATTT11.9607843137254901No Hit
CCTCTGGAACCTGTGAATGCGCCCTCATTTGGAAAAAATAATGCAGAGAT11.9607843137254901No Hit
GATTCTGAGGGAAGAGGACAAGCAGCAGATCTCAAAAGCACTCAAGCAGC11.9607843137254901No Hit
TTTCTCTGCTCTCCGGGAGCCCACAGTCTAGGTAGGAGAGAGCCATGTCT11.9607843137254901No Hit
GTTAATTTCTAACAAGCCAGACACATAAAACCTATGCTCTTCCCTGCAGG11.9607843137254901No Hit
GTTTAGTGGGTATAGACTTAGAACCAAAGCAACTCTTGGCTGTTTTTAAT11.9607843137254901No Hit
ACTACAGAGAAATACACATATTGACTTTGAATATTATGATCTGATGTTTG11.9607843137254901No Hit
CCTTTCTCTCCTAATTGTTTGATACACTTTTCATATCTCCAATTCATCTA11.9607843137254901No Hit
GTCAAAGTTTCAAAATATCTTTACGACTTATTTTACTATGATGGAGTTTA11.9607843137254901No Hit
CTCTCGGTCTAGACATAAAAGCAGCAAACCAAGATGGAAATCAAATTGTA11.9607843137254901No Hit
CTTTAATGGTTCCATTGGAATGTTTGTCATTTAGAAAGGCCCCTTGAGTC11.9607843137254901No Hit
CCAGATAGGCCTCCAAGCGCAGAGCTGTCGATGCGAGGAATAATCGTCAT11.9607843137254901No Hit
GTTGGTGTCAGTATATGTTTCTGACACTTTCATTAGACCACACTATCTCA11.9607843137254901No Hit
GCAGAAAAATCAAAATACGAAGTGTGAAAAAAAAAAAAAAGAGATCAGAT11.9607843137254901No Hit
TCCCATCATAATATTAAAAGTCAATATGTGTATTTATCTGTAGTTCATCC11.9607843137254901No Hit
GAATCAGGAAGGCTAATAGATTTCCTCAAGGATGTGATGGAATCAATGGA11.9607843137254901No Hit
GGCCAAGACCAACCCACAGTGTCACTGTTTACACCACAAAAGGATATGCT11.9607843137254901No Hit
GGGGTCTTTTCCATGATCGCCAGGAACAATCTTGCGATAACAGGCCCTCA11.9607843137254901No Hit
ACAGTTTCTTGACAATTCCTCAAGAGTCATGTCAGTTATGTATCGCGAAG11.9607843137254901No Hit
CTTGACATACGAAAATTCGGAAATTTTTTATGCATACTTACAATTATATG11.9607843137254901No Hit
GATATGATGGTTGGCATTAAGGCCGACTCAACACAACTGGTACCTATTGT11.9607843137254901No Hit
TTCATAACCTAAGCGTGACTACTCGGTGAGCCTTCTACAGTTCCACACCA11.9607843137254901No Hit
ACATATTATGCATAGACTCTCTTAATTACATTTCTCAGAAGCAGCTAAAG11.9607843137254901No Hit
AGTAAAGACATGGAATCAATCCAAATGCCCATCAATGATAGACTGGATAA11.9607843137254901No Hit
GGGTCTTATTACCGTGGGTATGTGTTTGTATACATGTAGTTTGTGGGGAG11.9607843137254901No Hit
CCATAGTATTACTAAGGGCTTTCACCGAAGAGGGCGCAATTGTTGGCGAA11.9607843137254901No Hit
CAACTTCCATAATTACATCTTGTGCCTCTTTGGCCCTGAGGTCTGCATGA11.9607843137254901No Hit
GGGAGTAACAAATCCACTACGAAAGTCACCAAACACATGGAAATGGTGGC11.9607843137254901No Hit
TATCAGAAACGAATGGGGGTGCAGATGCAACGATTCAAGTGACCCGCTTG11.9607843137254901No Hit
AACTAATACAAAGGTGTCAACTAGGGCCATAATCAAACAGTAAAACGCAT11.9607843137254901No Hit
CATCATAGGGGTAACAGTAGCTCTAGGCTTTCCAGCGTAAAACAAAAACG11.9607843137254901No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT11.9607843137254901No Hit
CCTCTGACCTCCCTCCTGAACGTCAGACGCATATATGCCCCTTCCTGGTC11.9607843137254901No Hit
ACATTTCCTCGAGGGTCATGTCAGAAAGGTAGCGCGAAGTAGGTACAGAT11.9607843137254901No Hit
CAATGCAACACAGTAAACACTTTCTAGAAAACTTTGTTTCCTGTACATAC11.9607843137254901No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC11.9607843137254901No Hit
GTTCTATAATAATGCATTTTAAGAGCATTTTTATTGAACCTGCACAATTA11.9607843137254901No Hit
AAATAGTACTGAAAGCGAACTTCAGTGAAATCGTTAACCGATTAGAGACC11.9607843137254901No Hit
GGCTAGCTCTATGTTGACAAACTGACAATCGTCAACATCCACCGCTCTCT11.9607843137254901No Hit
ATACCAATGAATGTCAACGTTTCTAAGTTACTATGGTCACGCAGTTTACG11.9607843137254901No Hit
ACCTGAGGTCAGGAATTCGAGAAAAGTAAAATCTTAAATTTCACCTTTTG11.9607843137254901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTG50.0145.02
TGATCAT50.0145.09
GTTTATA50.0145.0145
GTGATCA50.0145.08
GTGGTGT50.0145.03
TGGTGTG50.0145.04
GTGTGAT50.0145.06
CAGTGGT50.0145.01
GGTGTGA50.0145.05
TGTGATC50.0145.07
Produced by FastQC (version 0.11.5)