FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075612
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATAACACTGAAATTCGCTT32320.15571311015738973No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC31490.1517142895685706No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG31090.14978714711612767No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC26700.1286367587005664No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA22950.1105697982089138No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA21930.1056555849551843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA14900.077.859061
GAGCAAA66950.076.242571
AGCGAAA24100.066.1882861
GCAAAAG76950.064.828463
CGAAAGC26300.062.8586463
CAAAAGC79900.062.6164174
AGCAAAA80450.061.6446042
GCGAAAG27500.059.849172
AAAAGCA103350.048.6894535
AAGCAGG130150.045.736617
AAAGCAG141350.043.1918876
AGCAGGG70900.041.7233288
GGATACC7900.040.38426
TCGCTCC6900.039.9319425
AGTAGAA28450.039.757381
TAGAAAC41600.039.2151762
CGCTCCC7750.035.552316
GCTTTCG8550.034.769081
CATACCG1905.4296834E-934.344249
AGCGTCA1956.9921953E-933.465233
Produced by FastQC (version 0.11.5)