FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4292913
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG52850.079.027272
GAGCGAA22950.078.675841
CGAAAGC53450.078.413293
AGCGAAA58550.070.470951
GAGCAAA113750.067.701431
CAAAAGC153100.049.967414
AGCAAAA159950.047.645062
GCAAAAG162850.046.7976953
AAAAGCA193450.040.145365
AAGCAGG225050.039.6904147
AAAGCAG258600.036.6209226
GAAAGCA125250.034.6204764
AGCAGGG148100.029.6175528
CTTTCGC15500.025.2616352
GCTTTCG15450.024.8755191
TCGCTCC17250.023.1200225
TAGAAAC57800.023.082852
TTCGCTC17450.022.8547674
GCAGGGT57850.022.5591669
AGCAGGT69450.021.2964298
Produced by FastQC (version 0.11.5)