FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7685845
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC93450.089.237793
AGCGAAA95150.087.108241
GCGAAAG111100.074.993732
GAAAGCA214300.040.030674
AAGCAGG289700.035.669476
AAAGCAG325800.035.4119155
GAGCGAA30500.032.5718571
GGTGCGT26550.028.1268468
AGGTGCG29050.027.2035687
AGCAGGG202350.027.0871987
TACGTGC12900.025.2911367
GTGCGTT29750.025.10169
GCAGGGC66050.023.6001078
ATGCCGG36400.022.506817145
CGTAGAC21400.019.9925653
CAGGGCG19550.018.9136169
GTATATA114250.018.7235261
GAGCAAA150850.018.26671
ATACGTG18550.017.9822586
CAATACG21450.017.917564
Produced by FastQC (version 0.11.5)