FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6316894
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA39900.084.332141
GAGCAAA190350.073.0326841
GCAAAAG229750.059.7803883
AGCGAAA67950.059.3380661
AGCAAAA231100.059.1482932
CAAAAGC238000.057.708174
CGAAAGC72600.057.5334133
GCGAAAG76550.054.2800522
AAGCAGG338700.048.397557
AAAAGCA289100.047.8845675
AAAGCAG376650.045.071416
GCTTTCG24400.038.934041
AGCAGGG203550.036.9356428
TCGCTCC26600.034.622615
GTAACGC9400.030.0874081
CTTTCGC32650.028.872942
ATTTCGG10200.028.4313748
CGCTCCC32000.028.1001996
AGCAGGC104300.025.9971228
TAGAAAC84750.025.4125122
Produced by FastQC (version 0.11.5)