FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6924121
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG164370.2373875326557696No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA104180.15045953125313669No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA99650.14391718457837463No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG85910.12407351055823547No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA80820.11672239696562206No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT76590.11061331828256611No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC71950.10391210667751184No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT70000.10109586473142224No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA42900.087.727021
GAGCAAA183850.077.661431
AGCGAAA71300.064.784811
GCGAAAG72050.064.7129062
CGAAAGC75800.061.703183
CAAAAGC275850.051.4965934
AGCAAAA275000.050.996182
GCGAAGC7600.050.5684133
AAGCAGG344400.050.1652157
GCAAAAG290850.048.5166473
AAAAGCA331800.043.1626175
AGCAGGG221300.042.9496778
AAAGCAG413750.042.587816
GCAGGGT116550.041.8024649
GTAGAAA101400.040.2618521
TCGCTCC24550.040.1702425
TAGAAAC102000.039.028882
CTTTCGC25600.037.9558532
GCTTTCG27750.035.015891
GCAGGTA52050.034.2656759