FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971976
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6000.071.308721
GAGCAAA19500.058.7576451
GCGAAAG9000.056.4023782
AGCGAAA9550.052.394731
CGAAAGC9700.052.3321043
TCGCTCC4700.049.373515
GCTTTCG5150.045.0593221
CTTTCGC5250.044.2010422
CAAAAGC26150.042.151574
GCGAAGC700.001055383941.438483
CGCTCCC5450.041.2483946
GCAAAAG27400.040.493263
AGCAAAA27800.039.649772
AGTCGGC1303.6987512E-739.0377588
AGTAGAA16100.038.7359731
TTCGCTC6300.037.9852754
CTCCGAC1553.4708137E-837.41868145
TTTCGCT6600.037.3574183
GCAGTCG1406.5970016E-736.258676
AAAGCAG41750.036.12846
Produced by FastQC (version 0.11.5)