FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5659623
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT92630.16366814538706906No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA86400.15266034504418405No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC76030.13433756983459852No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT75390.13320675246390085No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC72790.12861280689544163No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG68590.12119181790023824No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG66920.11824109132357404No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA65980.11658020331036184No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG61970.10949492572208432No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG61030.10783403770887212No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC59890.10581976926731691No Hit
TCCTTACTCTGGGTCTAGATCCGATATTCGGGATTACAGCTTGTTGGCTT59800.10566074807456256No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG59120.1044592546181963No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA32400.063.556591
CGAAAGC27700.063.0853273
AGCGAAA33250.051.0283431
GCAAAAG119050.047.7504843
GAGCGAA21600.045.6533241
GCGAAAG40150.043.7024272
AAGCAGG156050.042.2802937
CAAAAGC153150.039.2490044
AGGGTGT25400.036.5405436
GAGCAAA166650.035.199011
CAGTACG16150.035.0172849
TAAACTA17550.034.7054065
TAGAAAC70000.034.2851222
AGCAGGT80800.033.1109478
GGTAGTT25600.032.286827
CGACTCG1153.1641388E-431.521414145
GTAGAAA78400.031.4448931
GCAGGGT41650.030.4637559
AGCAAAA195550.029.9962142
AGCGTCA3151.8189894E-1229.9242943
Produced by FastQC (version 0.11.5)