FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5471332
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC214320.39171448561337535No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT197900.3617035120515443No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA180140.32924340910037997No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC171200.3129036951148276No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT153600.2807360255235836No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT141570.25874869227456865No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG137610.2515109666165387No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA123990.22661757685331468No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA122860.2245522662488769No Hit
GTCATACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA122440.22378462867908583No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT121090.22131722220475747No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC118210.21605342172619024No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA118000.21566960294129472No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC117310.214408484076638No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG116680.21325702772195146No Hit
ATGCCCAAGCAGAAAATGGAAGGACCTCTTTGCATCAGAATGGACCAGGC111850.20442919566935436No Hit
CTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAATGTTATTT101290.185128593914608No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA94100.17198736980318505No Hit
CTTGAATGGAATGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATT90870.16608387134979197No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG85830.15687222051229938No Hit
CTTATCTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAATGT85300.15590353500756307No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT84760.15491657241783172No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT81960.14979898861922472No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA81050.14813577388467744No Hit
GTTCTTATCTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAA79140.14464485065062768No Hit
TCATTACTCATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTC78420.14332890053098588No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT74340.1358718498530157No Hit
TCCTAGTTCCAGTACTGGTTTAAGAGATGATCTTCTTGAAAATTTGCAGA73700.1347021164133341No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT73190.1337699850785878No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC71670.13099186815934402No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA70620.12907277423486638No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGT69220.12651398233556288No Hit
GTAATGAGAATGGGGGATCTCCACTTACTCCAAAACAGAAACGGGAAATG69090.12627638023062757No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG67020.1224930236366574No Hit
TTGTCATACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACT66400.12135984436696585No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG66040.12070186930714494No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT65560.11982456922738376No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTGTCGTTCCATCAGGCCCC64160.11726577732808026No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG64140.11722922315809021No Hit
GTTTTTAATCTGTGTCTCACTTCTTCAATTAGCCATCTTATCTCTTCAAA63840.11668091060823946No Hit
ATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACAATTGC63760.11653469392827927No Hit
TTTTTAATCTGTGTCTCACTTCTTCAATTAGCCATCTTATCTCTTCAAAC62680.11456076874881657No Hit
ATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTCAAC60020.10969906414013991No Hit
ATACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGC59870.10942490786521454No Hit
ACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGCTA58470.10686611596591104No Hit
GGTTTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT57970.10595226171615979No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG56260.10282688018201053No Hit
CCATAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA56020.10238823014212992No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT55860.10209579678220954No Hit
TCTTATCTCTTCAAACTTTTGACCTAGCTGTTCTCGCCATTTCCCGTTTC55370.10120021961745329No Hit
ATACTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTT55130.10076156957757271No Hit
GTACAAATTGCTTCAAATGAGAACATGGATAATATGGGATCGAGCACTCT54980.10048741330264734No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA19600.087.315611
CGAGTCG2200.075.795235145
AGCGAAA44150.074.240981
CGAAAGC44350.074.0663153
GCAAAAG98700.072.0722353
AGTAGAA41950.066.0339661
GCGAAAG51900.063.5711672
GCAGGGT67800.061.4874959
TAGAAAC76050.053.2048382
AGGGTGT64450.051.8673636
AGCAGGG101150.049.8870748
CAAAAGC147400.048.997964
ACAAGGG88400.046.2639773
CAAGGGT91450.044.641714
CAGTACG11100.042.4559979
AAGGGTG79500.042.322085
GAGCAAA179550.040.6705471
AGCAAAA179200.040.5053332
AGCAGGC21700.039.7586758
TCATACT47000.038.8793372
Produced by FastQC (version 0.11.5)