FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6064820
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA206700.34081802922428034No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC183220.3021029478203805No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG160920.26533351360798835No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT144920.23895185677398506No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT139610.2301964444122002No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC137680.22701415705659853No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT124920.20597478573148092No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA114520.18882670878937874No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC108390.17871923651485122No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC107410.1771033600337685No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG103030.1698813814754601No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA102300.1686777183824087No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA94300.15548688996540705No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA94260.15542093582332203No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT93860.15476139440247197No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA91790.15134826754957278No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG87650.14452201384377442No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC86970.1434007934283293No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG86040.14186735962485286No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT82300.13570064733990456No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG79610.13126523128468776No Hit
TCCTAGTTCCAGTACTGGTTTAAGAGATGATCTTCTTGAAAATTTGCAGA79580.131215765678124No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC78410.1292866070221375No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT76820.12666492987425843No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG75290.12414218393950685No Hit
GTCATACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA71140.11729944169818725No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG70930.11695318245224094No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG70900.11690371684567719No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATA69940.115320817435637No Hit
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT69210.1141171543425856No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA68750.113358681708608No Hit
CTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAATGTTATTT68310.1126331861456729No Hit
TCCTTACTCTGGGTCTAGATCCGATATTCGGGATTGCAGTTTGTTGGCTT67130.11068753895416518No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG65230.10755471720512728No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTCG4600.088.25941145
GCAAAAG116850.082.228393
GCAGGGG18450.080.163929
AGTAGAA24500.078.142421
GCAGGGT59850.072.440629
CGAAAGC31600.068.844443
GAGCGAA22350.068.4626851
AGCAGGC6400.061.172888
AGCGAAA37950.057.517991
AGGGTGT39250.056.7200976
CAAAAGC184250.053.919734
AGCAGGG114150.051.3827978
GCGAAAG46700.047.048532
TATATTC15200.046.276753
AAGCAGG244350.046.0498357
TAGAAAC62100.045.1897282
TTATATT15400.044.7325062
GAGCAAA219950.044.5760651
AGCAAAA224100.043.5535472
ACAAGGG61150.043.521543
Produced by FastQC (version 0.11.5)