FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4639639
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA117250.072.4233551
GCGAAAG51100.069.10732
AGCGAAA51650.067.531961
GCAAAAG156650.054.021383
CGAAAGC66450.053.5811163
GAGCGAA29950.053.509391
AGCAAAA159400.052.724322
CAAAAGC170750.049.6029324
AAAAGCA204750.041.7202225
AAAGCAG260450.038.3941576
AAGCAGG248200.038.236157
AGTAGAA72050.031.4018461
AGCAGGG143900.028.3153028
GAAAGCA143600.025.7032814
GTAGAAA92150.025.5754131
TAGAAAC94050.024.7497863
AACAAGG130150.023.5117072
AGCAGGC78200.022.9927338
TCGCTCC17150.021.9869235
CGCTCCC18350.020.1539126
Produced by FastQC (version 0.11.5)