FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4912697
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA133520.27178553857483984No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT107290.21839327766397967No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT94460.1922772766160828No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC91530.18631313919828557No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC90200.18360586862979744No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT88030.17918874296542203No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA83250.16945885325311127No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG82460.16785077524626493No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC79510.16184592699285139No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG73660.14993800757506517No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC72070.14670149614356431No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG71360.14525626147918344No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAGAAAAGACG70820.14415706891754163No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC67450.13729729311618447No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG66100.13454931171207996No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG65530.1333890528970136No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT65150.1326155470203027No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT63460.12917548141072002No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA62830.12789309008880456No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG58970.12003589881484651No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA57300.1166365440408802No Hit
CTATTGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA57160.11635156819156565No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA55640.11325754468472204No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC53850.10961392489705755No Hit
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT53040.10796513605459486No Hit
TTCTTAATCTGTGTCTCACTTCTTCAATTAGCCATCTTATCTCTTCAAAC52190.10623492554089943No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG52050.10594994969158489No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA51440.10470826920528581No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA51040.10389405249295856No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTC50850.1035072995546031No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA50240.10226561906830403No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA49800.10136998068474405No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTCG3100.084.19303145
AGTAGAA28350.080.573921
GAGCGAA20150.070.1772841
CGAAAGC32950.066.242883
GCAAAAG127800.063.5499733
GCAGGGT44900.061.359459
AGCGAAA35500.060.8730351
AGGGTGT30600.055.4526826
CAGTACG15800.051.393139
CAAAAGC164900.050.9232444
GCGAAAG43200.050.6918452
AAGCAGG193350.049.233457
TAAACTA12700.047.9627155
GCAGGGG19450.047.7127579
AGCAGGG83350.045.7530338
TAGAAAC57700.043.6082422
GAGCAAA189900.043.4946441
CAAGGGT48400.042.2505074
AGCAAAA196300.041.815862
CCTCGCG1755.2750693E-1141.428314145
Produced by FastQC (version 0.11.5)