FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6871948
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCACACCATCGATTTCTTTTACGATAACTTCTGGACGAGATACTTG171170.24908512113304698No Hit
CTCCAACAGACGCGGTTGAAGCTCTTCCAATCCTACACATCGATGAGCCA157590.22932362119154567No Hit
GAGCAAAAGCAGGAGAAGCGAATGTGTATCGTTTGGCAGAAGTTTCTGTA152960.22258608476082767No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG139340.2027663771611776No Hit
GAGCAAAAGCAGGTGTGTAGTTAAAACTAATACTCGGTGTCATCATATGT122220.17785349947351176No Hit
AAACAAGGATTTGCAAAAAAGGCTCCTTTTTTGTGGATTTTCCAAGCGGC121150.17629644461803262No Hit
CATTTCACACCATCGATTTCTTTTACAATAACCTCTGGACGAGATACTTG100750.14661053896216908No Hit
GAAATATACATTCCCATACCCACCAGAGGCAATTACTACAGCATGTGCAG99970.14547548962826842No Hit
GTTGAATATCCAATCAAACCACGCGCTGGAACAAGGAAGACCAAACGAGT96310.14014948890765763No Hit
GTGTAGCACAAGGGGTTCCTTTTGGTCGTGAATATGGCGGTCAGTTGGAT93710.13636599112798875No Hit
TTCCCATACCCACCAGAGGCAATTACTACAGCATGTGCAGAATGTCTCTC89330.1299922525607004No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA76270.11098745217513288No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA73000.10622897612147239No Hit
ATTCTAAAGTGAATAATTATAGCATACAATGGATACACACTCAAGATGCC71550.10411894851358014No Hit
ATATAATGGTACAAACGGGACTTAATTTCCGTAACGATGTGAATACCACA69110.10056828136650625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA44800.0102.135121
GAGCAAA223850.093.554321
GACGCGG49950.072.7183159
AGACGCG51600.070.6735158
CAAAAGC299350.069.665984
AAGCAGG393900.063.149517
AGCAAAA330800.062.887842
GCAAAAG333900.062.5007863
AAAAGCA350150.059.6623345
GCGAAAG101750.056.369442
CGAAAGC102250.056.165933
AAAGCAG450250.056.109396
TCACACC89050.054.557195
AGCGAAA106500.052.9728971
TTCACAC93300.052.4605944
AGCAGGT136800.052.1491788
ACCATCG93300.051.7528349
AGCAGGG217600.048.6776668
GCAGGGT80150.048.4844939
GGGTCAA118150.046.574318145
Produced by FastQC (version 0.11.5)