FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6593422
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA82800.078.729511
GCGAAAG86100.077.057152
CGAAAGC89150.074.178563
GAGCGAA33450.066.983921
GAGCAAA142600.066.96911
CAAAAGC201400.047.0926554
GCAAAAG210750.045.072183
AGCAAAA216250.043.99192
AAAAGCA271500.035.5211265
AAAGCAG351150.033.5556456
AAGCAGG336950.032.8350757
GAAAGCA223800.030.3587864
GTATAGG34350.026.1759911
AGCAGGG222250.024.3030138
TACCCCG28450.023.448255
CCGCTCA31250.021.5765429
GTAACGC10100.020.8201961
TCGAACG4551.5279511E-1020.7174784
GCTTTCG23050.020.447991
TCGCTCC24150.019.216215
Produced by FastQC (version 0.11.5)