FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4745110
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTTAAATGGTCTGTTTGGTAGCGAACAAGGCAAGAGATTCGTTAAAT70980.14958557335867873No Hit
GTATTGGATGTGTTCAAAAGATACCAAGAAGAATATGACAGGTATTTAAA64860.1366880852077191No Hit
CTCCAAGCCCGCGTAGCCGTGCAGTACGTCGAATTTTGCGCACAGGTAAT57580.12134597511964948No Hit
TTGTAGTAGTGGGTGGCAACCTGGTATTCGTGTGGCTCCCATTCATGATA50660.10676254080516573No Hit
TCGTAAAGGTGGTGCAGGCGGTTGGGAACAGCTTTGATGACCGGCCTTAA50420.1062567569561085No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG79550.060.4321862
GAGCAAA99200.054.019511
CGAAAGC98400.049.742383
GCAAAAG120400.044.086183
GAAAGCA129500.038.5804524
AGCAAAA144300.036.882872
AGCGAAA133600.035.279691
AAGCAGG187500.034.9235236
CAAAAGC154850.034.6528054
AGCAGGG95900.034.0217637
AAAGCAG229250.031.9789525
AGCAGGC62500.027.2618528
AAAAGCA223100.024.3447155
GCAGGGC49900.022.376278
ATGTGTT44650.021.9219828
TGGATGT57250.018.3660165
ATTGGAT63250.018.3432923
GGATGTG55250.017.856
GCAGGGA67850.017.6320029
GAGCGAA84900.017.1674651
Produced by FastQC (version 0.11.5)