FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n01_HK_S01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK_S01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4403066
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA132050.095.1587451
GAGCGAA26400.090.360891
GCGAAAG61300.070.732562
AGCGAAA60700.070.119361
CGAAAGC63750.068.470723
AGCAAAA180200.067.879552
CAAAAGC190250.065.248074
GCAAAAG204550.060.403023
AAAAGCA231150.054.3309755
AAAGCAG286650.051.1219986
AAGCAGG297350.048.9132927
AGCAGGG173200.036.4200258
AGCAGGC89050.034.8480648
TCGTGTA10350.034.3276674
TCGCTCC15850.032.480495
CGTGTAG11500.032.156285
TATCGTG13150.031.9801832
TAGAAAC73750.031.0673162
CGCTCCC16700.030.8272916
AGTAGAA71050.029.187071
Produced by FastQC (version 0.11.5)