FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7793452
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT463620.5948840128867157No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA268830.34494342173404036No Hit
GATGTATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGT256480.3290967853526268No Hit
CTAAAATAGATCTCTGGTCATACAACGCGGAGCTTCTTGTTGCCCTGGAG249990.3207692816995601No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA236850.30390897384111687No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG228360.29301521328417757No Hit
TCTAATAACAGTTTCTTTAGTAGATTGAATTGGTTGACCCACTTAAAATT221720.28449524036332036No Hit
GTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA209960.2694056497685493No Hit
GTTATTAGATCTCCTTTTGCAAGCAGAGCTTGTTCCATTTTGAGTGACTC207460.2661978286387085No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC206460.26491470018677216No Hit
ATCTGGGACAGGCCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTG185430.23793050884255143No Hit
CTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGC141970.18216574632139904No Hit
GTCCAATAGATGCTTATTCTGCTGGGGATATCCCTTACCCTGGGTCTAGA141350.18137020668119852No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT138780.17807256655972217No Hit
TCATAAGGGTAACAGTTGCTGTGGGCTTTGCTGCGTTCAACAAAAAGGTC134690.17282457119130265No Hit
TCTCTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCA131300.16847476573923853No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT130850.1678973579358672No Hit
CTATTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAG130800.16783320151327039No Hit
CTATTAACCATGAAGACTATCTTTGCTTTGAGCTACATTCTATGTCTGGT130180.16703766187306984No Hit
ACATTCTATGTCTGGTTTTCGCTCAAAAACTTCCCGGAAATGACAACAGC128920.1654209200236301No Hit
CCCTTACCCTGGGTCTAGATCCGATATTCGGGATTACAGTTTGTTGGCTT122260.1568752845337342No Hit
CTATTTTAGTGTCCTCAACATATTTCTCGAGGTCCTGAATTCTCCCTTCT122210.1568111281111374No Hit
ATTTTAGTGTCCTCAACATATTTCTCGAGGTCCTGAATTCTCCCTTCTAC121820.15631070801488225No Hit
CTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAAGAAATGGGACCT121440.15582311920314643No Hit
ATTGTATGTTGGTTCTCCAGGGCAACAAGAAGCTCCGCGTTGTATGACCA118850.15249981651263136No Hit
CATATGGGGCCTGTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCA118250.15172993944146956No Hit
ACATACAATTGATCTAACTGACTCAGAAATGAACAAACTGTTTGAAAGAA118150.15160162659627593No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT117760.1511012065000208No Hit
GTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCAACAGGGATGCGA117120.15028000429078153No Hit
TAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAGGGAATCTAATT116270.14918934510663567No Hit
TCTCAAAAGCACTCAAGCAGCAATCAACCAAATCAATGGGAAGCTGAATA112440.14427496313571958No Hit
ATCTATTAGTGTGCAGTTTTCTCCATCAAGGATCTGATGAGGACTGTCGC111530.14310731624445752No Hit
CACTAAAATAGATCTCTGGTCATACAACGCGGAGCTTCTTGTTGCCCTGG110370.1416188872402114No Hit
GTTTTACTATTGTCCAATAGATGCTTATTCTGCTGGGGATATCCCTTACC109960.14109280457491752No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT109510.14051539677154617No Hit
GTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGT109180.14009196438240717No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCTTT107890.13843672867940934No Hit
GCATTAATGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGC106570.13674299912285337No Hit
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCG105940.1359346281981335No Hit
ATGTGATCCTGTTTACATTTTGAAATGGTTTGTCATTGGGAATGCTTCCA105750.1356908337922656No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT100230.12860796473757713No Hit
GTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATA99060.12710670444881164No Hit
CCCTTATGATGTGCCGGATTATGCCTCCCTTAGGTCACTAGTTGCCTCAT98360.12620851453245624No Hit
ACTAAAGAAACTGTTATTAGATCTCCTTTTGCAAGCAGAGCTTGTTCCAT98190.12599038269562707No Hit
GGTATATCTTTCGCTCCGAGTCGGCATTTTGGGCCGCCGGGTTATTAGTA95730.1228338867038637No Hit
TCTATTAGTGTGCAGTTTTCTCCATCAAGGATCTGATGAGGACTGTCGCA91920.11794516730198633No Hit
GTCCGTACCCGGGTGGTGAACTCCCCAAATGTACAATTTGTCAAAATTTT91440.11732926564505691No Hit
ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT91220.1170469773856309No Hit
ATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG90930.11667487013456937No Hit
ATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGTTGAGC90260.11581517407177205No Hit
GGATCACATATGGGGCCTGTCCCAGATATGTTAAGCAAAACACTCTGAAA89300.11458337075791318No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG88730.11385198754030948No Hit
ATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTT87450.11220958312183099No Hit
CACTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAAT86830.11141404348163048No Hit
ATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGC85530.10974597649411326No Hit
TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC84630.10859116088737057No Hit
GGGTAAGGGATATCCCCAGCAGAATAAGCATCTATTGGACAATAGTAAAA84410.1083088726279446No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT82550.10592225370734303No Hit
TTCTATGTCTGGTTTTCGCTCAAAAACTTCCCGGAAATGACAACAGCACG81300.10431834314242265No Hit
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGA80940.10385641689972556No Hit
TTCTTTAGTAGATTGAATTGGTTGACCCACTTAAAATTCAAATACCCAGC80530.10333033423443166No Hit
GATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT80160.10285557670721523No Hit
ATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT79870.1024834694561537No Hit
GATGAGGACTGTCGCATATTCCACCTGTTGAGGAACTCTGAACCAGCTCA79840.10244497560259562No Hit
GTCCTGGGGTTGACCAGAGGGCCCCGGGCGCTCCGTGTGTGGCTGCGATG79040.10141847284104656No Hit
GATCTAACTGACTCAGAAATGAACAAACTGTTTGAAAGAACAAAGAAGCA78760.10105919687450439No Hit
ACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATA78700.1009822091673882No Hit
TACTAATGCTACTGAGCTGGTTCAGAGTTCCTCAACAGGTGGAATATGCG78520.10075124604603967No Hit
GGTACGGACAATGACCAAATCAGCCTATATGCTCAAGCATCAGGAAGAAT78490.10071275219248159No Hit
CCCTCAGTGTGATGGCTTCCAAAATAAGAAATGGGACCTTTTTGTTGAAC78260.10041763264853623No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGAC9700.076.993343
TTTCGGA10000.073.9579852
TCGGACA10450.072.161364
CGGACAT11600.064.382365
GACATCT11500.064.304697
GGACATC12150.061.4683276
CTTTCGG12650.059.613471
CCGTTCC13150.050.1704145
AAGCCCG8500.033.264236145
ATTTGTA8950.033.211826145
CATGGTC224500.033.1017239
GCTAGCT5050.033.0235754
TATACAT231150.032.560292
TACATCA230500.032.5264974
ATCATGG229600.032.5241247
ATACATC235250.031.9313853
ACATCTG23550.031.7092938
TAGCTTG5800.030.0036246
TATATAT127350.029.5497112
GTATATA128950.029.0154911
Produced by FastQC (version 0.11.5)