FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7720001
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCG265400.34378233888829807No Hit
CTAGTGATCCGGCCATGGCACGTGGGTGCGTGGTCGCTCAACGGATAAAA157370.2038471238539995No Hit
GAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCGTGGTC155740.20173572516376617No Hit
TACTAGGGACGGCCCTTCTCAAATTTCCTGCGCGCACAGAGGATAGGGAC155500.20142484437502017No Hit
GGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACG114060.14774609485154214No Hit
TAGCCAACCAGCCATGCACCTGGCGGTACAACTGGCACACCAGAGGTTCG107030.13863987841452352No Hit
GATAAAAGGTACCCCGGGGATAACAGGCTGATCTTGCCCAAGAGTCCATA96520.12502589054068775No Hit
GTATGGAGGATGGCGTAATCGTAAGATGCTTGGCTTCTATGAAAATCTTT93660.12132122780813112No Hit
GTTTAATTTTGAGGATGGTTATTTTTATCCAGCTAAGGAAAGCATCGATA91970.11913210892071129No Hit
AAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCGAACGT90990.11786267903333174No Hit
GAACTAGTGATCCGGCCATGGCACGTGGGTGCGTGGTCGCTCAACGGATA84360.10927459724422316No Hit
GGTCAATACAACCCCATGTTGTGTAGCCCCAAAGCTCTACACCATCTTCA80000.10362692958200395No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA222250.084.247841
AAAAGCA289000.065.366775
GCAAAAG290800.064.78763
AGCAAAA295500.063.48432
CAAAAGC337450.056.0675744
AAGCAGG382850.055.4686787
CGAAAGC104800.053.765893
GCGAAAG108350.051.935012
AGTAGAA88800.049.651791
GCAGGCT115250.049.2587669
GAGCGAA74600.048.7988051
AGCAGGC167100.045.471788
AAAGCAG514750.043.7859276
AGCGAAA132700.042.0242231
GGTCGAT22300.040.6489641
ACAAGGC72050.035.2201428
GTAGAAA143050.034.6239931
ATTTCCG21750.033.332638145
TAGTGCG21050.032.376727
AGTGCGC22000.030.9786348
Produced by FastQC (version 0.11.5)