FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2383753
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA49560.20790744678664272No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT34320.14397464838009644No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG34110.1430936846225259No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA33250.13948592828199902No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT33100.13885666845516292No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT32490.1362976784926962No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA30020.12593586667746196No Hit
ATTCTACACTGTAGAGACCCATTAGAACACATCCAGAAACTGATTGCCCC29980.12576806405697236No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT29270.12278956754328155No Hit
GCAGAGGCCATGGAGGTTGCTAATCAGACTAGGCAGATGGTACATGCAAT24830.1041634766689334No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT24800.10403762470356619No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG24510.10282105570501641No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA24080.10101717753475296No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT23970.10055572032840651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9650.0108.218211
CGAAAGC28900.077.5383763
GCGAAAG29750.075.808932
GAGCAAA62200.074.153541
AGCGAAA33450.067.209521
CAAAAGC94500.049.1138764
AAGCAGG108250.048.1577227
AGTAGAA32950.047.5404741
GCAAAAG98650.047.2682953
AGCAAAA101200.045.646332
GTAGAAA41400.045.3695641
TAGAAAC41550.045.0293432
CAATTCG9050.043.2614489
AAAAGCA111650.041.9603355
GCAGGGT25950.041.629849
GAAAGCA56150.040.812524
AAAGCAG144150.039.2917676
GCAGGTA20800.035.9029169
ACAAGGG37600.035.1026273
GACAGTA11600.034.3771327
Produced by FastQC (version 0.11.5)