FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5179828
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG241890.4669846180220656No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA143080.2762253881789125No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA115390.2227680146908353No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA106000.2046399996293313No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT91840.17730318458450745No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG89580.17294010534712737No Hit
GTACTATGTTCTTTTCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGG89260.17232232421617089No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC85230.16454214309818782No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT82910.1600632298987534No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT79740.15394333557021583No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC76030.14678093558318925No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT75830.14639482237634147No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT69060.1333248903245436No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA64910.12531304128245185No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC63470.12253302619314774No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT63360.12232066392938144No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG60680.11714674695762098No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC60400.11660618846803408No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT55440.10703058093820875No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG53530.10334319981281231No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT52650.10164430170268202No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA27600.0111.667511
GAGCAAA176450.073.484011
GCGAAAG50200.063.4154742
CGAAAGC51650.061.6381533
AGCAAAA223250.057.8504682
AAGCAGG272550.057.77997
GCAGGTA49550.055.750499
GCAGGGT119450.053.5971769
AGCAGGG166000.052.7626048
GTAGAAA56450.051.7711871
GCAAAAG260350.049.581293
AGGCTCG5150.049.270893145
CAAAAGC266150.048.746034
CGCGAAA1255.1932147E-946.4103052
AAAGCAG343000.046.4087646
TAGAAAC63700.045.4221732
GCGAAGC4650.045.2272763
AGTAGAA43950.044.3854261
AGTCGGC1801.8189894E-1244.3085488
AGCGAAA74000.042.7269361
Produced by FastQC (version 0.11.5)