FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434833
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA51130.35634809068372414No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG39230.27341160957407584No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT32300.22511330586904538No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC31940.22260430307917367No Hit
GTTCATTGATGCTTAATGCTGGTCCGTACCTTCTGTCTTCCTTACCTATA29760.20741089729606166No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC26800.18678131880156087No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA26730.1862934571479747No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC24960.1739575267644388No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT24520.17089096779904003No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC23530.16399121012689283No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA23200.16169129090284373No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG21450.1494947495631896No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA19680.13715881917965364No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT18930.1319317300340876No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC18610.1297015053319794No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT18550.12928333820033414No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT17980.1253107504497039No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC17700.12335930383535923No Hit
GTCATACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA17270.12036243939190136No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA17190.11980488321637431No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC16990.11841099277755669No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG16700.11638985164127115No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT16350.11395054337334032No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA16340.11388084885139943No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG16280.11346268171975415No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG15340.10691139665731135No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTACAAAATAA15180.10579628430625725No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG15180.10579628430625725No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG14880.10370544864803083No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT14570.10154491846786351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8400.0104.450181
AGGCTCG1550.084.194435145
GCAAAAG45950.077.797313
AGTAGAA8050.070.258981
CAAAAGC55250.065.751944
GAGCAAA54950.065.451131
CGAAAGC14700.063.631993
AGCGAAA14850.062.5009571
AGCAGGC8350.060.7790878
AGCAAAA60700.058.7732282
AGTCGGC651.0938171E-555.769828
GCAGGGT24200.055.7237329
GCGAAAG16900.055.3485372
AAGCAGG80300.055.3461657
AGCAGGG34400.054.3755768
AGCGTCA553.2170917E-452.735183
GCGCAAG553.2170917E-452.735182
GCAGTCG701.6948536E-551.793486
TAGAAAC20500.050.9344672
CGTGTAG1457.2759576E-1250.00755
Produced by FastQC (version 0.11.5)