FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3652747
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTATAGTGATTCGTTTCCCAAGATAAAAAGCCGCTATGTAAGCATCT74220.20318954474536563No Hit
GTATCAGTACACGGCACAGGCAGAAAACAGCCTGCAAATCACCCTACCGA66190.1812060895539713No Hit
GTATATGTGTATTATTACGGCAATGCTAAACGACACAAAGAGGCTCTTAC45520.12461854051211321No Hit
CACATATACTTTGCCGTCTTTTTCTGTTGAAATAGGCGAGAGCCCTTTGA44950.12305807109005908No Hit
CTATAGAGAAAGCTAAACAAATAGAAAAATAGAACCTATAAATAAAAACT44910.12294856446395No Hit
CTATAATACTCATTCTTGCAGTCGTGAATACACAGGCACAGGAACGACAA41980.11492720410146116No Hit
GTATACTTATCGAGACGGCCTTTCTTACTAGTAACGAAGAGCGAAAGTTC40180.10999940592655337No Hit
ATCTTACAAAGTGCAAATTTCGTCTAGCCCCAAACGTTTGGAAGCTAAGC39730.10876745638282641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG80950.043.444292
CGAAAGC82750.042.3246043
AGCGAAA88200.039.54651
GAAAGCA102650.035.5322574
GAGCAAA74300.032.012281
AAGCAGG128700.032.003356
AGCAAAA91700.025.4621072
AAAGCAG202600.024.1238215
AGCAGGG61050.024.1078627
AGCAGGC49200.023.7251577
TCTATAG68950.022.4005723
CAAAAGC105900.022.11674
TATAGTG65750.021.8371055
TATTATA74600.020.8009322
CTATAGT67750.020.5497074
GCAAAAG125000.019.723443
GTACACG50400.019.7078117
ATATGTG45850.018.8200763
TACACGG52800.018.8122588
TAACGTG15300.018.4841215
Produced by FastQC (version 0.11.5)