FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAGCACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC13.7037037037037033No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC13.7037037037037033No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGT13.7037037037037033No Hit
CACATGTTGCACATACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCC13.7037037037037033No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC13.7037037037037033No Hit
TCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAAGA13.7037037037037033No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCGT13.7037037037037033No Hit
GTTTAGTGTTGTTCCCTGTCCAAGGGCAAATTGATAACACTTGTCAGGAT13.7037037037037033No Hit
GACAGCAGCTGCACAAAGAGCAATGGTGGATCAAGTGAGAGAAAGTCGGA13.7037037037037033No Hit
CTCATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCT13.7037037037037033No Hit
CAGCACGGCAACGCTGTGCCTTGGGCACCATGCAGTACCAAACGGAACGA13.7037037037037033No Hit
GCTGGGTATTTGAAGTTTAAGTGGGTCAACCAATTCAATCTACTAAAGAA13.7037037037037033No Hit
TACCAGAGGACAAGAGCTCTTGTTCGAACTGGAATGGATCCCAGAATGTG13.7037037037037033No Hit
CATCCCTGTTGCCAATTTCAGAGTGTTTTGCTTAACATATCTGGGACAGG13.7037037037037033No Hit
CATAAATGGGAGAAATACTGTGTCCTTGAGATAGGAGATATGTTACTAAG13.7037037037037033No Hit
TCGCATGACACATAAGGTTCTCTTGTCACCCAGATGTCCCCACCAGCGGA13.7037037037037033No Hit
ACTTCAAACAGCAGTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCT13.7037037037037033No Hit
ATACTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTT13.7037037037037033No Hit
GTTTAATGCTTCATCTCTGTATACATCGTGGTCATAAGTTCCATTTCTGA13.7037037037037033No Hit
CTTCACATCCCTGAAGTCTGCTTAAAATGGGAGCTGATGGATGAGAATTA13.7037037037037033No Hit
CCACAATATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAACAAGCG13.7037037037037033No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG13.7037037037037033No Hit
AAATCATTATGTGAGTTAGACCAGATGTAGCATCCTCACCATTGTTGGCT13.7037037037037033No Hit
ACACAGGTGGTGGTTGTCAACACTGTCTAGAAGGAGTGTACAGAAAGAGA13.7037037037037033No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCAGTCTCGCACT13.7037037037037033No Hit
GAATGATGCAACATACCAGAGGACAAGAGCTCTTGTTCGAACTGGAATGG13.7037037037037033No Hit
GACAGGTGCTGTGTTAGGGTGAGGAGAAAGTGCCTCTTGATTTGGTTACA13.7037037037037033No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)