FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4637640
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCG231080.4982706721522154No Hit
GAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCGTGGTC147240.31748906771547597No Hit
TACTAGGGACGGCCCTTCTCAAATTTCCTGCGCGCACAGAGGATAGGGAC139340.3004545415340561No Hit
CTAGTGATCCGGCCATGGCACGTGGGTGCGTGGTCGCTCAACGGATAAAA97670.21060280659990857No Hit
TAGCCAACCAGCCATGCACCTGGCGGTACAACTGGCACACCAGAGGTTCG90440.19501298074020407No Hit
GATAAAAGGTACCCCGGGGATAACAGGCTGATCTTGCCCAAGAGTCCATA65920.14214126150369583No Hit
GAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCG65790.14186094651590034No Hit
GGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACG58670.12650831026125356No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA56950.1227995273458052No Hit
GCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCGAA55320.1192848086526768No Hit
AAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCGAACGT50590.10908565563519376No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG49850.1074900164738962No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT48020.10354404395339009No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC46530.10033120293942609No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA180450.094.276331
AGCAAAA202250.082.748492
GCAAAAG216300.077.676863
AAAAGCA236150.071.4247365
CAAAAGC237750.071.004294
CGTCGAC2050.067.196557
GAGCGAA58500.066.6895751
ATTTCCG11400.062.960506145
AAGCAGG327450.061.1762927
AGCAGGC107150.059.7463078
GCAGGCT87150.056.81939
AAAGCAG365850.056.331056
GCGAAAG74500.053.240712
AGCGAAA75800.051.7558671
AGTAGAA48650.051.2751731
CGAAAGC79700.049.950083
AGCAGGG117000.042.8188258
GTAGAAA87350.038.686061
AGCAGGA152550.037.5929878
ACGTCGA4300.035.4142236
Produced by FastQC (version 0.11.5)